Cytoprotective effects of fermented oyster extracts against oxidative stress-induced DNA damage and apoptosis through activation of the Nrf2/HO-1 signaling pathway in MC3T3-E1 osteoblasts

Authors

  • Cheol Park Division of Basic Sciences, College of Liberal Studies, Dong‐eui University, Busan, Republic of Korea
  • Hyesook Lee Anti‐Aging Research Center, Dong‐eui University, Busan, Republic of Korea; Department of Biochemistry, Dong‐eui University College of Korean Medicine, Busan, Republic of Korea
  • Min Ho Han National Marine Biodiversity Institute of Korea, Seocheon, Republic of Korea
  • Jin-Woo Jeong Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources, Sangju, Republic of Korea
  • Sung Ok Kim Department of Food Science and Biotechnology, College of Engineering, Kyungsung University, Busan, Republic of Korea
  • Soon-Jeong Jeong Department of Dental Hygiene, College of Health Science, Youngsan University, Yangsan, Republic of Korea
  • Bae‐Jin Lee Ocean Fisheries & Biology Center, Marine Bioprocess Co., Ltd., Busan, Republic of Korea
  • Gi‐Young Kim Department of Marine Life Science, Jeju National University, Jeju, Republic of Korea
  • Eui Kyun Park Department of Oral Pathology and Regenerative Medicine, School of Dentistry, Kyungpook National University, Daegu, Republic of Korea
  • You‐Jin Jeon Department of Marine Life Science, Jeju National University, Jeju, Republic of Korea
  • Yung Hyun Choi Department of Biochemistry, Dong-eui University College of Korean Medicine, 52-57, Yangjeong-ro, Busan 47227, Republic of Korea. Tel: 82-51-890-3319; Fax: +82-51-890-3333; E-mail: choiyh@deu.ac.kr; Anti‐Aging Research Center, Dong‐eui University, Busan, Republic of Korea

DOI:

https://doi.org/10.17179/excli2020-2376

Keywords:

fermented oyster extract, ROS, DNA damage, apoptosis, Nrf2/HO-1

Abstract

Osteoblast damage by oxidative stress has been recognized as a cause of bone-related disease, including osteoporosis. Recently, we reported that fermented Pacific oyster (Crassostrea gigas) extracts (FO) inhibited osteoclastogenesis and osteoporosis, while promoting osteogenesis. However, since the beneficial potential of FO on osteoblasts is not well known, in the present study, we investigated the cytoprotective effect of FO against oxidative stress in MC3T3-E1 osteoblasts. Our results demonstrated that FO inhibited hydrogen peroxide (H2O2)-induced DNA damage and cytotoxicity through the rescue of mitochondrial function by blocking abnormal ROS accumulation. FO also prevented apoptosis by suppressing loss of mitochondrial membrane potential and cytosolic release of cytochrome c, decreasing the rate of Bax/Bcl-2 expression and reducing the activity of caspase-9 and caspase-3 in H2O2-stimulated MC3T3-E1 osteoblasts, suggesting that FO protected MC3T3-E1 osteoblasts from the induction of caspase dependent- and mitochondria-mediated apoptosis by oxidative stress. In addition, FO markedly promoted the activation of nuclear factor-erythroid-2-related factor 2 (Nrf2), which was associated with the enhanced expression of heme oxygenase-1 (HO-1). However, inhibiting the expression of HO-1 by artificially blocking the expression of Nrf2 using siRNA significantly eliminated the protective effect of FO, indicating that FO activates the Nrf2/HO-1 signaling pathway in MC3T3-E1 osteoblasts to protect against oxidative stress. Based on the present data, FO is thought to be useful as a potential therapeutic agent for the inhibition of oxidative stress in osteoblasts.

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Published

2020-08-04

How to Cite

Park, C., Lee, H., Han, M. H., Jeong, J.-W., Kim, S. O., Jeong, S.-J., … Choi, Y. H. (2020). Cytoprotective effects of fermented oyster extracts against oxidative stress-induced DNA damage and apoptosis through activation of the Nrf2/HO-1 signaling pathway in MC3T3-E1 osteoblasts. EXCLI Journal, 19, 1102–1119. https://doi.org/10.17179/excli2020-2376

Issue

Vol. 19 (2020)

Section

Original articles

License

Copyright (c) 2020 Cheol Park, Hyesook Lee, Min Ho Han, Jin-Woo Jeong, Sung Ok Kim, Soon-Jeong Jeong, Bae‐Jin Lee, Gi‐Young Kim, Eui Kyun Park, You‐Jin Jeon, Yung Hyun Choi

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