High carotenoid production by a halotolerant bacterium, Kocuria sp. strain QWT-12 and anticancer activity of its carotenoid

Authors

  • Zahra Rezaeeyan Extremophiles Laboratory, Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran
  • Atefeh Safarpour Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran 19395-4644, Iran
  • Mohammad Ali Amoozegar Extremophiles Laboratory, Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran
  • Hamid Babavalian Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
  • Hamid Tebyanian Division of Tissue Engineering and Regenerative Medicine, Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran; Research Center for Prevention of Oral and Dental Disease, Baqiyatallah University of Medical Sciences, Tehran, Iran
  • Fatemeh Shakeri Division of Tissue Engineering and Regenerative Medicine, Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

DOI:

https://doi.org/10.17179/excli2017-218

Keywords:

halotolerant bacteria, Kocuria, yellow pigmented bacteria, carotenoid, cancer cell line

Abstract

Halophilic prokaryotes are extremophile microorganisms that grow optimally in media containing salts and almost appeared pigmented. Many of them contain high concentrations of carotenoids. Amongst 15 strains of halophilic prokaryotes isolated from industrial tannery wastewater in Qom, a Gram-stain-positive coccoid, aerobic, non-endospore-forming, halotolerant bacterium designated as strain QWT-12 showed high capacity in the production of carotenoids in a wide range of culture medium factors. 16S rRNA gene analysis showed that this strain belongs to the genus Kocuria. Carotenoids from this strain were extracted by methanol. MTT assay for extracted carotenoid was carried out against seven cancer cell lines belonging to breast, lung and prostate cancer with negative control of fibroblast cell line through six concentration levels to find out IC50. Based on statistical analysis of data from MTT assay, IC50 of 1, 4 and 8 mg/ml for MCF-7, (A549 and MDA-MB-468) and MDA-MB-231 respectively. Additionally, qualitative carotenoid determination was carried out using spectrophotometric method in 300-600 nm and thin layer chromatography, respectively. According to the obtained results from mass spectrophotometry, absorption spectrum of strain QWT-12 is similar to the absorption spectrum of the carotenoid neurosporene.

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Published

2017-06-12

How to Cite

Rezaeeyan, Z., Safarpour, A., Amoozegar, M. A., Babavalian, H., Tebyanian, H., & Shakeri, F. (2017). High carotenoid production by a halotolerant bacterium, Kocuria sp. strain QWT-12 and anticancer activity of its carotenoid. EXCLI Journal, 16, 840–851. https://doi.org/10.17179/excli2017-218

Issue

Vol. 16 (2017)

Section

Original articles

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