Broad-spectrum antimicrobial activity of wetland-derived Streptomyces sp. ActiF450

Authors

  • Mabrouka Benhadj Department of Applied Biology, Faculty of Exact Sciences and Natural and Life Sciences, Larbi Tebessi University, 12002 Tebessa, Algeria; Biomolecules and Application Laboratory, Faculty of Exact Sciences and Natural and Life Sciences, Larbi Tebessi University, 12002 Tebessa, Algeria
  • Roumaisa Metrouh Department of Applied Biology, Faculty of Exact Sciences and Natural and Life Sciences, Larbi Tebessi University, 12002 Tebessa, Algeria
  • Taha Menasria Department of Applied Biology, Faculty of Exact Sciences and Natural and Life Sciences, Larbi Tebessi University, 12002 Tebessa, Algeria; E-mail: tahamenasria@hotmail.com https://orcid.org/0000-0003-4925-6165
  • Djamila Gacemi-Kirane Department of Biochemistry, Faculty of Science, University Badji Mokhtar Annaba, Annaba, 23000, Algeria
  • Fatma Zohra Slim Department of Applied Biology, Faculty of Exact Sciences and Natural and Life Sciences, Larbi Tebessi University, 12002 Tebessa, Algeria
  • Stephane Ranque Aix Marseille University, IRD, APHM, SSA, VITROME, IHU-Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13005 Marseille, France https://orcid.org/0000-0003-3293-5276

DOI:

https://doi.org/10.17179/excli2020-1124

Keywords:

coastal wetland, Streptomyces, antifungal activity, Candida spp.

Abstract

The increased incidence of invasive infections and the emerging problem of drug resistance particularly for commonly used molecules have prompted investigations for new, safe and more effective microbial agents. Actinomycetes from unexplored habitats appear as a promising source for novel bioactive compounds with a broad range of biological activities. Thus, the present study aimed to isolate effective wetland-derived actinomycetes against major pathogenic fungi and bacteria. Water samples were collected from various locations of Fetzara Lake, Algeria. Thereafter, an actinomycete designated ActiF450 was isolated using starch-casein-agar medium. The antimicrobial potential of the newly isolated actinomycete was screened using the conventional agar cylinders method on Potato Dextrose Agar (PDA) against various fungal and bacterial pathogens. A wetland-derived Streptomyces sp. Actif450 was identified as Streptomyces malaysiensis based on its physiological properties, morphological characteristics, and 16S rDNA gene sequence analysis. The antimicrobial activity of Streptomyces sp. ActiF450 showed a potent and broad spectrum activity against a range of human fungal pathogens including moulds and yeasts, such as Arthroderma vanbreuseghemii, Aspergillus fumigatus, A. niger, Candida albicans, C. glabarta, C. krusei, C. parapsilosis, Fusarium oxysporum, F. solani, Microsporum canis, Rhodotorula mucilaginous and Scodapulariopsis candida. In addition, high antibacterial activity was recorded against pathogenic staphylococci. The novel Streptomyces sp. ActiF450 may present a promising candidate for the production of new bioactive compounds with broad-spectrum antimicrobial activity.

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Published

2020-03-12

How to Cite

Benhadj, M., Metrouh, R., Menasria, T., Gacemi-Kirane, D., Slim, F. Z., & Ranque, S. (2020). Broad-spectrum antimicrobial activity of wetland-derived Streptomyces sp. ActiF450. EXCLI Journal, 19, 360–371. https://doi.org/10.17179/excli2020-1124

Issue

Vol. 19 (2020)

Section

Original articles

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