Antiviral activity of flavonoids present in aerial parts of Marcetia taxifolia against Hepatitis B virus, Poliovirus, and Herpes Simplex Virus in vitro

Authors

  • Joseph Thomas Ortega Laboratorio de Virología Molecular, Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas, Caracas, Venezuela; Cátedra de Virología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina
  • María Luisa Serrano Unidad de Química Medicinal, Facultad de Farmacia, Universidad Central de Venezuela, Caracas, Venezuela
  • Alírica Isabel Suárez Laboratorio de Productos Naturales, Facultad de Farmacia, Universidad Central de Venezuela, Caracas, Venezuela
  • Jani Baptista Laboratorio de Productos Naturales, Facultad de Farmacia, Universidad Central de Venezuela, Caracas, Venezuela
  • Flor Helene Pujol Laboratorio de Virología Molecular, Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas, Caracas, Venezuela
  • Lucía Vicenta Cavallaro Cátedra de Virología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina
  • Héctor Rodolfo Campos Cátedra de Virología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina; E-mail: rcampos@ffyb.uba.ar
  • Héctor Rafael Rangel Laboratorio de Virología Molecular, Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas, Caracas, Venezuela. Tel: 58 212 5041874; E-mail: hrangel@ivic.gob.ve; hrangel2006@gmail.com

DOI:

https://doi.org/10.17179/excli2019-1837

Keywords:

antiviral, flavonoids, Marcetia taxifolia, HBV, HSV, polio

Abstract

Marcetia taxifolia is a neotropical plant present in South America and it has been evaluated in several biological models due to the presence of active metabolites. Nevertheless, there is a limited quantity of studies related to the antiviral activity of the compounds present in this genus. In our work, the antiviral effect of the compounds isolated from the aerial parts of Marcetia taxifolia was evaluated against Hepatitis B virus (HBV), Herpes Simplex Virus type 1 (HSV-1), and Poliovirus type 1 (PV-1). The cytopathic effect and viral quantification by qPCR were determined as indicative of antiviral activity. Our data show that myricetin rhamnoside (MyrG), myricetin-3-α-O-ramnosil (1→6)-α-galactoside (MyrGG), 5,3'-dihydroxy-3,6,7,8,4’-pentamethoxyflavone (PMF), 5-hydroxy-3,6,7,3',4'pentamethoxyflavone (PMF-OH) had antiviral activity without cytotoxic effects. The methoxyflavones PMF and PMF-OH were the most active compounds, showing an antiviral effect against all the evaluated viruses. Computational studies showed that these compounds could interact with the Reverse Transcriptase. Altogether, these results suggest that the flavonoids (related to myricetin and methoxyflavones) are the main antiviral compounds present in the aerial parts of Marcetia taxifolia. Furthermore, our results showed that the methoxyflavones have a broad antiviral activity, which represents an opportunity to evaluate these flavonoids as lead molecules to develop new antiviral compounds.

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Published

2019-11-05

How to Cite

Ortega, J. T., Serrano, M. L., Suárez, A. I., Baptista, J., Pujol, F. H., Cavallaro, L. V., … Rangel, H. R. (2019). Antiviral activity of flavonoids present in aerial parts of Marcetia taxifolia against Hepatitis B virus, Poliovirus, and Herpes Simplex Virus in vitro. EXCLI Journal, 18, 1037–1048. https://doi.org/10.17179/excli2019-1837

Issue

Vol. 18 (2019)

Section

Original articles

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