Immunoregulatory, proliferative and anti-oxidant effects of nanocurcuminoids on adipose-derived mesenchymal stem cells

Authors

  • Forouzan Yousefi Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
  • Fahimeh Lavi Arab Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
  • Mahmoud Reza Jaafari Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Pharmaceutical Nanotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
  • Maryam Rastin Immunology Research Center, BuAli Research Institute, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  • Nafiseh Tabasi Immunology Research Center, BuAli Research Institute, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  • Mahdi Hatamipour Nanotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
  • Karim Nikkhah Department of Neurology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  • Mahmoud Mahmoudi Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Immunology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Tel: +98 9151156304, Fax: +98 5138022229, E-mail: MahmoudiM@mums.ac.ir

DOI:

https://doi.org/10.17179/excli2019-1366

Keywords:

immunoregulatory, antioxidant, nanocurcuminoids, adipose-derived mesenchymal stem cells

Abstract

Curcuminoids are dietary complexes extracted from the seeds of Curcuma longa L. that contain curcumin, bisdemethoxycurcumin and desmethoxycurcumin. Curcuminoids are popular for their pleiotropic therapeutic functions, such as their anti-inflammatory and anti-oxidant effects. Nonetheless, their clinical use is associated with poor systemic bioavailability and insolubility. The nano-formulation of curcuminoids eliminates these shortcomings. In the present study, we explored immunoregulatory, proliferative and anti-oxidant effects of nanocurcuminoids on adipose-derived mesenchymal stem cells (AT-MSCs). Flow cytometry analysis and MTT assay were employed to explore the effects of nanocurcuminoids on the apoptosis and proliferation of adipose-derived MSCs (AT-MSCs). The anti-oxidant effect of nanocurcuminoids on AT-MSCs also was examined. The immune regulatory effect of nanocurcuminoids was evaluated by the flow cytometric measurement of the T regulatory (Treg) population. The expression of inflammatory and anti-inflammatory cytokines was quantified using real-time PCR.  Our findings demonstrate that low concentrations of nanocurcuminoids are beneficial for MSC proliferation, protection of MSCs from apoptosis, reducing inflammatory cytokines and SOD activity. A high concentration of nanocurcuminoids increases the population of Tregs and elevates the expression of TGFβ and FOXP3 genes. The beneficial effects of nanocurcuminoids on AT-MSCs were mainly observed at low doses of nanocurcuminoids.

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Published

2019-06-17

How to Cite

Yousefi, F., Lavi Arab, F., Jaafari, M. R., Rastin, M., Tabasi, N., Hatamipour, M., … Mahmoudi, M. (2019). Immunoregulatory, proliferative and anti-oxidant effects of nanocurcuminoids on adipose-derived mesenchymal stem cells. EXCLI Journal, 18, 405–421. https://doi.org/10.17179/excli2019-1366

Issue

Vol. 18 (2019)

Section

Original articles

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