Evaluation of microsatellite instability in tumor and tumor marginal samples of sporadic colorectal cancer using mononucleotide markers

Authors

  • Jafar Nouri Nojadeh Department of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
  • Shahriar Hashemzadeh Department of General and Thoracic Surgery, Tabriz University of Medical Sciences, Tabriz, Iran; Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  • Hossein Samadi Kafil Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  • Shahin Behrouz Sharif Department of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Molecular Medicine, Pasteur Institute of Iran, Tehran, Iran
  • Amirtaher Eftekharsadat Department of Pathology, Imam Reza Hospital, Tabriz University of Medical Sciences, Tabriz, Iran
  • Tohid Ghasemnejad Department of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
  • Mortaza Ghojazadeh Liver and Gastrointestinal Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  • Ebrahim Sakhinia Department of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Connective Tissue Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

DOI:

https://doi.org/10.17179/excli2018-1455

Keywords:

CRC, MSI, DNA MMR system

Abstract

Microsatellite instability (MSI) is a unique molecular alteration that is due to a defective DNA mismatch repair (MMR) system. Approximately, 15-20 % of sporadic colorectal cancers (CRC) display MSI. Determination of MSI status in CRC has prognostic and predictive implications. Additionally, detecting MSI is used diagnostically for tumor detection and classification. The present study analyzed a panel of five mononucleotide markers, BAT-25, BAT-26, NR-21, NR-22 and NR-27, amplified in a single multiplex PCR reaction to evaluate MSI status in CRC patients. Genomic DNA from 50 CRC and paired adjacent normal tissues was used for PCR-based MSI analysis. Our finding showed microsatellite instability in 36 % of specimens. Instability with differences in allele lengths was observed in the tumoral DNA compared to the tumor-free margin DNA sample. The frequency of instability in NR-21, BAT-26 and BAT-25 markers were more than others; their frequency were 35.48 %, 29.03 %, and 22.58 %, respectively. In conclusion, the NR-21, BAT-26, and BAT-25 were the most useful markers for discriminating cancer tissue from normal, therefore these markers have demonstrated promising potential for determining MSI status in patients with sporadic colorectal cancer.

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Published

2018-09-24

How to Cite

Nouri Nojadeh, J., Hashemzadeh, S., Samadi Kafil, H., Behrouz Sharif, S., Eftekharsadat, A., Ghasemnejad, T., … Sakhinia, E. (2018). Evaluation of microsatellite instability in tumor and tumor marginal samples of sporadic colorectal cancer using mononucleotide markers. EXCLI Journal, 17, 945–951. https://doi.org/10.17179/excli2018-1455

Issue

Vol. 17 (2018)

Section

Original articles

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