Leukemia cell microvesicles promote survival in umbilical cord blood hematopoietic stem cells

Authors

  • Farnaz Razmkhah Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
  • Masoud Soleimani Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
  • Davood Mehrabani Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  • Mohammad Hossein Karimi Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  • Sedigheh Amini Kafi-abad Department of Pathology, Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran

DOI:

https://doi.org/10.17179/excli2015-101

Keywords:

microvesicles, cell survival, leukemia, hematopoietic stem cells, P53 gene expression

Abstract

Microvesicles can transfer their contents, proteins and RNA, to target cells and thereby transform them. This may induce apoptosis or survival depending on cell origin and the target cell. In this study, we investigate the effect of leukemic cell microvesicles on umbilical cord blood hematopoietic stem cells to seek evidence of apoptosis or cell survival. Microvesicles were isolated from both healthy donor bone marrow samples and Jurkat cells by ultra-centrifugation and were added to hematopoietic stem cells sorted from umbilical cord blood samples by magnetic associated cell sorting (MACS) technique. After 7 days, cell count, cell viability, flow cytometry analysis for hematopoietic stem cell markers and qPCR for P53 gene expression were performed. The results showed higher cell number, higher cell viability rate and lower P53 gene expression in leukemia group in comparison with normal and control groups. Also, CD34 expression as the most important hematopoietic stem cell marker, did not change during the treatment and lineage differentiation was not observed. In conclusion, this study showed anti-apoptotic effect of leukemia cell derived microvesicles on umbilical cord blood hematopoietic stem cells.

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Published

2015-03-13

How to Cite

Razmkhah, F., Soleimani, M., Mehrabani, D., Karimi, M. H., & Kafi-abad, S. A. (2015). Leukemia cell microvesicles promote survival in umbilical cord blood hematopoietic stem cells. EXCLI Journal, 14, 423–429. https://doi.org/10.17179/excli2015-101

Issue

Vol. 14 (2015)

Section

Original articles

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