Regenerative potential of partially differentiated mesenchymal stromal cells in a mouse model of a full-thickness skin wound

Authors

  • Ausra Liubaviciute State Research Institute Centre for Innovative Medicine, Department of Stem Cell Biology, Santariskiu str. 5, LT-08406 Vilnius, Lithuania http://orcid.org/0000-0001-7316-1305
  • Vytautas Kaseta State Research Institute Centre for Innovative Medicine, Department of Stem Cell Biology, Santariskiu str. 5, LT-08406 Vilnius, Lithuania
  • Aida Vaitkuviene State Research Institute Centre for Innovative Medicine, Department of Stem Cell Biology, Santariskiu str. 5, LT-08406 Vilnius, Lithuania
  • Zygmunt Mackiewicz State Research Institute Centre for Innovative Medicine, Department of Regenerative Medicine, Santariskiu str. 5, LT-08406 Vilnius, Lithuania
  • Gene Biziuleviciene State Research Institute Centre for Innovative Medicine, Department of Stem Cell Biology, Santariskiu str. 5, LT-08406 Vilnius, Lithuania

DOI:

https://doi.org/10.17179/excli2018-1504

Keywords:

mesenchymal stromal cells, partial differentiation, keratinocyte-conditioned medium, skin regeneration

Abstract

Mesenchymal stromal cells (MSCs, known as mesenchymal stem cells) are considered to be a promising therapeutic tool for many diseases. But it is still unclear which cells are more efficient and safe for wound healing and tissue regeneration for clinical applications: undifferentiated, partially differentiated stem cells or differentiated cells. In this study, we modified MSCs with keratinocyte-conditioned medium (KCM) and examined MSCs, partially differentiated MSCs (PMSCs) and differentiated cell migration, accumulation in the wounded area as well as cell regenerative efficiency in a full-thickness skin wound model. In addition to that, the impact of intradermal and intravenous cell delivery methods of wound healing was evaluated. C57BL/6J mouse compact bone MSCs were treated with a KCM for 14 days. Flow cytometry analysis showed the appearance of keratinocyte surface markers which were absent in MSCs, whereas the specific markers for MSCs were lost. Cells were injected either intravenously or intradermally in C57BL/6J mice. Wound closure, cell migration and accumulation in the wounded area were further analysed. Wound healing was assessed by the rate of wound closure and by histological evaluation. Cells were monitored using optical imaging. We demonstrated that PMSCs showed morphology similar to keratinocyte cells, had enhanced migration and increased survival at the site of injury. PMSCs had a beneficial effect on wound healing and tissue regeneration. This effect was reinforced when these cells were injected intravenously. Due to their partial differentiation status, we assume that PMSCs can differentiate more rapidly into epidermal cell lineages thus causing faster and qualitatively improved wound healing.

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Published

2018-08-31

How to Cite

Liubaviciute, A., Kaseta, V., Vaitkuviene, A., Mackiewicz, Z., & Biziuleviciene, G. (2018). Regenerative potential of partially differentiated mesenchymal stromal cells in a mouse model of a full-thickness skin wound. EXCLI Journal, 17, 871–888. https://doi.org/10.17179/excli2018-1504

Issue

Vol. 17 (2018)

Section

Original articles

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