Exploiting in vitro potential and characterization of surface modified Zinc oxide nanoparticles of Isodon rugosus extract: Their clinical potential towards HepG2 cell line and human pathogenic bacteria

Authors

  • Aisha Siddiquah Department of Biotechnology, Quaid-i-Azam University, Islamabad-45320, Pakistan
  • Syed Salman Hashmi Department of Biotechnology, Quaid-i-Azam University, Islamabad-45320, Pakistan
  • Sadaf Mushtaq Department of Biotechnology, Quaid-i-Azam University, Islamabad-45320, Pakistan
  • Sullivan Renouard Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), UPRES EA 1207, Université d'Orléans, Chartres, France
  • Jean Philippe Blondeau Conditions Extrêmes et Matériaux: Haute Température et Irradiation (CEMHTI) CNRS UPR3079, 1D avenue de la Recherche Scientifique, 45071 Orléans, France
  • Rashda Abbasi Institute of Biomedical & Genetic Engineering (IBGE), Sector G-9/1, Islamabad, Pakistan
  • Christophe Hano Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), UPRES EA 1207, Université d'Orléans, Chartres, France
  • Bilal Haider Abbasi Department of Biotechnology, Quaid-i-Azam University, Islamabad-45320, Pakistan; Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), UPRES EA 1207, Université d'Orléans, Chartres, France

DOI:

https://doi.org/10.17179/excli2018-1327

Keywords:

nanotoxicity, ZnONP, pH, surface adaptation, characterization, Isodon rugosus

Abstract

Little is known about biogenically synthesized Zinc oxide nanoparticles (ZnONPs) from Isodon rugosus. Synthesis of metal oxide NPs from essential oil producing medicinal plants results in less harmful side effects to the human population as compared to chemically synthesized NPs. In this article, we report biogenic synthesis of ZnONPs from in vitro derived plantlets and thidiazuron (TDZ) induced callus culture of Isodon rugosus. Synthesized NPs were characterized using UV-spectra, XRD, FTIR, SEM and EDX. Furthermore, the NPs were evaluated for their potential cytotoxic (against HepG2 cell line) and antimicrobial (against drug resistant Staphylococcus epidermidis, Bacillus subtilis, Klebsiella pneumoniae and Pseudomonas aeruginosa) activities. Pure crystalline ZnONPs with hexagonal and triangular shapes were obtained as a result of callus extract (CE) and whole plant extract (WPE), respectively. ZnONPs showed potent cytotoxic and antimicrobial potential. The antimicrobial and cytotoxic activities of ZnONPs were found to be shape and surface bound phytochemicals dependent. CE mediated hexagonal ZnONPs showed superior anti-cancer and antimicrobial activities as compared to WPE mediated triangular shaped ZnONPs. It is concluded that biogenic ZnONPs have incredible potential as theranostic agents and can be adopted as useful drug delivery system in next generation treatment strategies.

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Published

2018-07-16

How to Cite

Siddiquah, A., Hashmi, S. S., Mushtaq, S., Renouard, S., Blondeau, J. P., Abbasi, R., … Abbasi, B. H. (2018). Exploiting in vitro potential and characterization of surface modified Zinc oxide nanoparticles of Isodon rugosus extract: Their clinical potential towards HepG2 cell line and human pathogenic bacteria. EXCLI Journal, 17, 671–687. https://doi.org/10.17179/excli2018-1327

Issue

Vol. 17 (2018)

Section

Original articles

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