Survivin polymorphisms and susceptibility to prostate cancer: A genetic association study and an in silico analysis

Authors

  • Mohammad Karimian Gametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran
  • Younes Aftabi Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran
  • Tahereh Mazoochi Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran
  • Faezeh Babaei Gametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran
  • Tahereh Khamechian Department of Pathology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran
  • Hossein Boojari Department of Statistics, Faculty of Sciences, Islamic Azad University, Kashan Branch, Kashan, Iran
  • Hossein Nikzad Gametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran

DOI:

https://doi.org/10.17179/excli2018-1234

Keywords:

prostate cancer, survivin gene, genetic polymorphism, in silico analysis

Abstract

Survivin is a member of the apoptosis inhibitor protein family and its polymorphisms may lead to susceptibility to cancer. The aim of this study was to investigate the possible association of c.-31G>C (rs9904341), c.454G>A (rs2071214), c.*148T>C (rs2239680) and c.*571T>C (rs1042489) polymorphisms of survivin gene with prostate cancer risk and provide some justification using in silico analysis. The 157 men with prostate cancer and 145 healthy controls were included in a case-control study. The studied polymorphisms were genotyped using PCR-RFLP method. An in silico approach was employed to show the possible effects of the polymorphisms on the survivin gene function. The study revealed that there are significant associations between c.-31CC genotype (OR= 2.29, 95 % CI= 1.20-4.37, p= 0.012), c.-31C allele (OR= 1.62, 95 % CI= 1.17-2.26, p= 0.004), c.454AG genotype (OR= 2.03, 95 % CI= 1.02-4.04, p= 0.043), and c.*148C allele (OR= 1.49, 95 % CI= 1.04-2.15, p= 0.031) with prostate cancer. Using stratified analysis, we found also significant effects of age distribution on the association of c.-31G>C with prostate cancer risk (OR= 2.10, 95 % CI= 1.08-4.10, p= 0.030). Also as a preliminary study, it was shown that smoking status has significant effects on the association of c.-31G>C (OR= 1.94, 95 % CI= 1.08-3.49, p= 0.027) and c.*148T>C (OR= 2.60, 95 % CI= 1.47-4.60, p= 0.001) polymorphisms with prostate cancer risk. Finally, in silico analysis revealed that c.-31G>C, which is located in a CpG island of the promoter may change transcriptional regulation of survivin gene and c.454G>A and *148T>C could affect protein structure and possible miRNA interaction with 3'-UTR of survivin transcript respectively. According to the results, c.-31G>C, c.454G>A, and c.*148T>C polymorphisms could be genetic risk factors for prostate cancer in an Iranian population. However, further studies with larger sample size and different ethnicities are required to obtain more comprehensive results.

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Published

2018-05-18

How to Cite

Karimian, M., Aftabi, Y., Mazoochi, T., Babaei, F., Khamechian, T., Boojari, H., & Nikzad, H. (2018). Survivin polymorphisms and susceptibility to prostate cancer: A genetic association study and an in silico analysis. EXCLI Journal, 17, 479–491. https://doi.org/10.17179/excli2018-1234

Issue

Vol. 17 (2018)

Section

Original articles

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