Determination of the relationship between doxorubicin resistance and Wnt signaling pathway in HeLa and K562 cell lines

Authors

  • Pelin Mutlu Central Laboratory Molecular Biology and Biotechnology R&D, Middle East Technical University, Ankara, Turkey
  • Serap Yalçin Azarkan Department of Molecular Biology and Genetics, Ahi Evran University, Kirsehir, Turkey
  • Negar Taghavi Pourianazar Department of Biological Sciences, Middle East Technical University, Ankara, Turkey
  • Meral Yücel Central Laboratory Molecular Biology and Biotechnology R&D, Middle East Technical University, Ankara, Turkey; Department of Biological Sciences, Middle East Technical University, Ankara, Turkey
  • Ufuk Gündüz Department of Biological Sciences, Middle East Technical University, Ankara, Turkey

DOI:

https://doi.org/10.17179/excli2018-1129

Keywords:

Wnt signaling pathway, drug resistance, cervical cancer, chronic myelogenous leukemia, gene expression

Abstract

Activation of the Wnt signaling in some types of cancer and its relation with chemotherapy resistance is a very interesting issue that has been emphasized in recent years. Although, it is known that increase in the activity of β-catenin is important in blast transformation and drug resistance, the underlying mechanisms are still unclear. In this study, changes in the expression levels of 186 genes that are thought to be important in drug resistance and Wnt signaling pathways were determined by using qPCR method in doxorubicin-sensitive and -resistant HeLa and K562 cell lines. It has been observed that the genes involved in the Wnt signaling pathways are involved in more changes in HeLa/Dox cells (36 genes) than in the K562/Dox cells (17 genes). Genes important for the development of cancer resistance have been found to be significantly different in expression levels of 18 genes in HeLa/Dox cells and 20 genes in K562/Dox cells. In both cell lines, the expression of ABCB1 gene was significantly increased to 160 and 103 fold, respectively. However, despite the resistance to same drug in HeLa and K562 cell lines, it appears that the expression levels of different oncogenes and genes involved in Wnt signaling pathways have been altered. It has been found that although resistance develops to the same drug in both cell lines, the expression levels of different genes have changed. If functional analysis of these genes is performed on patient population groups, these molecules may become candidates for novel therapeutic target molecules.

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Published

2018-05-02

How to Cite

Mutlu, P., Yalçin Azarkan, S., Taghavi Pourianazar, N., Yücel, M., & Gündüz, U. (2018). Determination of the relationship between doxorubicin resistance and Wnt signaling pathway in HeLa and K562 cell lines. EXCLI Journal, 17, 386–398. https://doi.org/10.17179/excli2018-1129

Issue

Vol. 17 (2018)

Section

Original articles

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