Protective effects of hydrogen sulfide on chronic kidney disease by reducing oxidative stress, inflammation and apoptosis

Authors

  • Hassan Askari Department of Physiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Behjat Seifi Department of Physiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Mehri Kadkhodaee Department of Physiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Nima Sanadgol Department of Biology, Faculty of Sciences, University of Zabol, Zabol, Iran; Young Researchers and Elite Club, Zahedan Branch, Islamic Azad University, Zahedan, Iran
  • Mohammed Elshiekh Department of Physiology, Faculty of Medicine, University of Dongola, Dongola, Sudan
  • Mina Ranjbaran Department of Physiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Parisa Ahghari Department of Physiology, Faculty of Medicine, Hamedan University of Medical Sciences, Hamedan, Iran

DOI:

https://doi.org/10.17179/excli2017-711

Keywords:

chronic kidney disease, hydrogen sulfide, oxidative stress, apoptosis, inflammation, creatinine clearance, blood pressure

Abstract

The current study aimed to examine the renoprotective effects of long-term treatment with sodium hydrosulfide (NaHS), a prominent hydrogen sulfide donor, in 5/6 nephrectomy animal model. Twenty-four rats were randomly divided into 3 groups including sham-operated group (Sham), 5/6-nephrectomized group (5/6 Nx), and NaHS-treated group (5/6Nx+NaHS). NaHS (30 micromol/l) was added twice daily into the drinking water and renal failure was induced by 5/6 nephrectomy. Twelve weeks after surgical procedure, blood pressure, creatinine clearance (CCr), urine concentration of neutrophil gelatinase associated lipocalin (NGAL) and tissue concentration of malondialdehyde (MDA), superoxide dismutase (SOD), as well as renal morphological changes, apoptosis (cleaved caspase-3) and inflammation (p-NF-κB) were measured. Five-sixth nephrectomy induced severe renal damage as indicated by renal dysfunction, hypertension and significant histopathological injury which were associated with increased NGAL and MDA levels, oxidant/antioxidant imbalance, decreased SOD activity and CCr and also overexpression of p-NF-κB and cleaved caspase-3 proteins. Instead, NaHS treatment attenuated renal dysfunction through reduction of NGAL concentration, hypertension, CCr, oxidant/antioxidant imbalance, inflammation and apoptosis. These findings suggest that long term NaHS treatment can be useful in preventing the progression of CKD by improving oxidant/antioxidant balance and reducing inflammation and apoptosis in the kidney.

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Published

2018-01-02

How to Cite

Askari, H., Seifi, B., Kadkhodaee, M., Sanadgol, N., Elshiekh, M., Ranjbaran, M., & Ahghari, P. (2018). Protective effects of hydrogen sulfide on chronic kidney disease by reducing oxidative stress, inflammation and apoptosis. EXCLI Journal, 17, 14–23. https://doi.org/10.17179/excli2017-711

Issue

Vol. 17 (2018)

Section

Original articles

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