Anti-cancer activities of Brassica juncea leaves in vitro

Authors

  • Youngeun Kwak Department of Food and Nutrition, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju, 28644, Korea
  • Jungjae Lee Department of Food and Nutrition, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju, 28644, Korea
  • Jihyeung Ju Department of Food and Nutrition, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju, 28644, Korea

DOI:

https://doi.org/10.17179/excli2016-586

Keywords:

mustard leaf, human cancer cell, growth, apoptosis, angiogenesis, metastasis

Abstract

Mustard (Brassica juncea) leaves are commonly consumed in different Asian and African countries. Cancer is a major burden of disease worldwide, and the colorectal and lung cancers are the leading cause of morbidity and mortality among cancers. In the current study, we aimed to investigate the effects of ethanol extract of mustard leaf (MLE) on the growth, angiogenic, and metastatic potentials of HCT116 colorectal carcinoma and H1299 non-small cell lung carcinoma cells in vitro. Treatment of HCT116 and H1299 cells with MLE inhibited cell growth in a dose-dependent manner (in the range of 175-700 µg/ml, by 39-86 %) and anchorage-independent colonization (at 700 µg/ml, by 56-86 %). Induction of apoptosis by MLE was evidenced by heterogeneous and condensed nucleus morphology, increased 4′,6-diamidino-2-phenylindole dihydrochloride staining intensity, and elevated sub-G1 cell population. In both HCT116 and H1299 cells, treatment with MLE markedly suppressed the secretion of key pro-angiogenic factors, such as vascular endothelial cell growth factor (by >92 %) and basic fibroblast growth factor (by 73-94 %). MLE was also effective in inhibiting critical events during metastasis, such as invasion (by 18-33 % in HCT116 and H1299), migration (45-82 % in H1299), and adhesion (by 17-45 % in HCT116 and H1299). These results indicate that MLE possesses in vitro anti-cancer activities against colon and lung cancers. It needs to be verified whether similar effects are reproduced in vivo.

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Published

2016-11-15

How to Cite

Kwak, Y., Lee, J., & Ju, J. (2016). Anti-cancer activities of Brassica juncea leaves in vitro. EXCLI Journal, 15, 699–710. https://doi.org/10.17179/excli2016-586

Issue

Vol. 15 (2016)

Section

Original articles

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