Keyhole limpet hemocyanin induces innate immunity via Syk and Erk phosphorylation

Authors

  • Kyoko Yasuda Laboratory of Marine Biochemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • Hideki Ushio Laboratory of Marine Biochemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo

DOI:

https://doi.org/10.17179/excli2016-488

Keywords:

keyhole limpet hemocyanin, innate immunity, NF-kappaB, Syk, Erk

Abstract

Hemocyanin is an extracellular respiratory protein containing copper in hemolymph of invertebrates, such as Mollusk and Arthropod. Keyhole limpet hemocyanin (KLH) is one of hemocyanins and has many years of experience for vaccine developments and immunological studies in mammals including human. However, the association between KLH and the immune systems, especially the innate immune systems, remains poorly understood. The aim of this study is to clarify the direct effects of KLH on the innate immune systems. KLH activated an inflammation-related transcription factor NF-κB as much as lipopolysaccharide (LPS) in a human monocytic leukemia THP-1 reporter cell line. We have found that the KLH-induced NF-κB activation is partially involved in a spleen tyrosine kinase (Syk) pathway. We have also successfully revealed that an extracellular signal-regulated kinase (Erk), a member of mitogen-activated protein kinases, is located in an upstream of NF-κB activation induced by KLH. Furthermore, a Syk phosphorylation inhibitor partially suppressed the Erk activation in KLH-stimulated THP-1. These results suggest that both Syk and Erk associate with the KLH-induced NF-κB activation in the human monocyte.

Downloads

Published

2016-08-03

How to Cite

Yasuda, K., & Ushio, H. (2016). Keyhole limpet hemocyanin induces innate immunity via Syk and Erk phosphorylation. EXCLI Journal, 15, 474–481. https://doi.org/10.17179/excli2016-488

Issue

Vol. 15 (2016)

Section

Original articles

License

Authors who publish in this journal agree to the following terms:

  • The authors keep the copyright and grant the journal the right of first publication under the terms of the Creative Commons Attribution license, CC BY 4.0. This licencse permits unrestricted use, distribution and reproduction in any medium, provided that the original work is properly cited.
  • The use of general descriptive names, trade names, trademarks, and so forth in this publication, even if not specifically identified, does not imply that these names are not protected by the relevant laws and regulations.
  • Because the advice and information in this journal are believed to be true and accurate at the time of publication, neither the authors, the editors, nor the publisher accept any legal responsibility for any errors or omissions presented in the publication. The publisher makes no guarantee, express or implied, with respect to the material contained herein.
  • The authors can enter into additional contracts for the non-exclusive distribution of the journal's published version by citing the initial publication in this journal (e.g. publishing in an institutional repository or in a book).