Anti-inflammatory effect of supercritical extract and its constituents from Ishige okamurae

Authors

  • Eun-Yi Ko Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea; Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea
  • Weon-Jong Yoon Jeju Biodiversity Research Institute, Jeju Technopark, Jeju, 699-943, Republic of Korea
  • Hae-Won Lee World Institute of Kimchi, Gwangju 503-360, Republic of Korea
  • Soo-Jin Heo Jeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science & Technology (KIOST), Jeju, 63349, Republic of Korea
  • Young-Hwan Ko Department of Food Bioengineering, Jeju National University, Jeju 690-756, Republic of Korea
  • I.P. Shanura Fernando Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea
  • Kichul Cho Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea
  • Chi-Heon Lee Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea; Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea
  • Sung-Pyo Hur Jeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science & Technology (KIOST), Jeju, 63349, Republic of Korea
  • Su-Hyeon Cho Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea; Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea
  • Ginnae Ahn Department of Marine Bio-Food Sciences, Chonnam National University, Yeosu 550-74, Republic Korea
  • Daekyung Kim Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea
  • Kil-Nam Kim Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea; Department of Marine Biotechnology, University of Science and Technology, Daejeon 305-350, Republic of Korea

DOI:

https://doi.org/10.17179/excli2016-337

Keywords:

supercritical fluid extraction, linoleic acid, Ishige okamurae, anti-inflammatory, NF-kappaB

Abstract

The anti-inflammatory properties of the supercritical fluid extract of Ishige okamurae (SFEIO) on lipopolysaccharide (LPS)-stimulated murine RAW 264.7 macrophages. The lipid profile of the SFEIO, reviled the presence of palmitic acid (220.2 mg/g), linoleic acid (168.0 mg/g), and oleic acid (123.0 mg/g). SFEIO was found to exert it's anti-inflammatory effects through inhibiting nitric oxide (NO), prostaglandin E2 (PGE2), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 production in LPS-stimulated RAW 264.7 cells, without inducing cytotoxicity. SFEIO did not effect on the LPS-induced p38 kinase phosphorylation, whereas it attenuated the extracellular-related signaling kinase (ERK) and c-Jun N-terminal kinase (JNK) phosphorylation. Furthermore, SFEIO inhibited the LPS-induced IκB-α degradation and p50 NF-κB activation. These results suggest that SFEIO exerts its anti-inflammatory effects in LPS-activated RAW 264.7 cells by down-regulating the activation of ERK, JNK, and NF-κB.

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Published

2016-06-28

How to Cite

Ko, E.-Y., Yoon, W.-J., Lee, H.-W., Heo, S.-J., Ko, Y.-H., Fernando, I. S., … Kim, K.-N. (2016). Anti-inflammatory effect of supercritical extract and its constituents from Ishige okamurae. EXCLI Journal, 15, 434–445. https://doi.org/10.17179/excli2016-337

Issue

Vol. 15 (2016)

Section

Original articles

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