Evaluation of Glucose-6-Phosphate Dehydrogenase stability in stored blood samples

Authors

  • Norunaluwar Jalil Department of Pathology, Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur; Department of Medical Laboratory Technology, Universiti Teknologi MARA, Puncak Alam, Selangor
  • Raja Zahratul Azma Department of Pathology, Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur
  • Emida Mohamed Department of Medical Laboratory Technology, Universiti Teknologi MARA, Puncak Alam, Selangor
  • Azlin Ithnin Department of Pathology, Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur
  • Hafiza Alauddin Department of Pathology, Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur
  • Siti Noor Baya Department of Pathology, Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur
  • Ainoon Othman Department of Medical Sciences II, Faculty of Medicine, Universiti Sains Islam Malaysia, Kuala Lumpur

DOI:

https://doi.org/10.17179/excli2015-604

Keywords:

G6PD stability, G6PD enzyme assay, OSMMR2000-D G6PD Assay Kit, EDTA, dried blood spot

Abstract

Glucose-6-Phosphate Dehydrogenase (G6PD) deficiency is the commonest cause of neonatal jaundice in Malaysia. Recently, OSMMR2000-D G6PD Assay Kit has been introduced to quantitate the level of G6PD activity in newborns delivered in Universiti Kebangsaan Malaysia Medical Centre (UKMMC). As duration of sample storage prior to analysis is one of the matters of concern, this study was conducted to identify the stability of G6PD enzyme during storage. A total of 188 cord blood samples from normal term newborns delivered at UKMMC were selected for this study. The cord bloods samples were collected in ethylene-diamine-tetra-acetic acid (EDTA) tubes and refrigerated at 2-8 °C. In addition, 32 out of 188 cord blood samples were spotted on chromatography paper, air-dried and stored at room temperature. G6PD enzyme activities were measured daily for 7 days using the OSMMR2000-D G6PD Assay Kit on both the EDTA blood and dried blood samples. The mean value for G6PD activity was compared between days of analysis using Student Paired T-Test. In this study, 172 out of 188 cord blood samples showed normal enzyme levels while 16 had levels corresponding to severe enzyme deficiency. The daily mean G6PD activity for EDTA blood samples of newborns with normal G6PD activity showed a significant drop on the fourth day of storage (p < 0.005) while for samples with severely deficient G6PD activity, significant drop was seen on third day of storage (p = 0.002). Analysis of dried cord blood showed a significant reduction in enzyme activity as early as the second day of storage (p = 0.001). It was also noted that mean G6PD activity for spotted blood samples were lower compared to those in EDTA tubes for all days (p = 0.001). Thus, EDTA blood samples stored at 2-8 °C appeared to have better stability in terms of their G6PD enzyme level as compared to dried blood samples on filter paper, giving a storage time of up to 3 days.

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Published

2016-02-19

How to Cite

Jalil, N., Azma, R. Z., Mohamed, E., Ithnin, A., Alauddin, H., Baya, S. N., & Othman, A. (2016). Evaluation of Glucose-6-Phosphate Dehydrogenase stability in stored blood samples . EXCLI Journal, 15, 155–162. https://doi.org/10.17179/excli2015-604

Issue

Vol. 15 (2016)

Section

Original articles

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