Partial purification and characterization of an antimicrobial activity from the wood extract of mangrove plant Ceriops decandra

Authors

  • Aritra Simlai Department of Biotechnology, Visva-Bharati University, Santiniketan, West Bengal, India
  • Kalishankar Mukherjee Department of Chemistry, Visva-Bharati University, Santiniketan, West Bengal, India
  • Anurup Mandal Division of Microbiology, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India
  • Kashinath Bhattacharya Department of Botany, Visva-Bharati University, Santiniketan, West Bengal, India
  • Amalesh Samanta Division of Microbiology, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India
  • Amit Roy Department of Biotechnology, Visva-Bharati University, Santiniketan, West Bengal, India

DOI:

https://doi.org/10.17179/excli2015-741

Keywords:

antimicrobial activity, bioactivity guided fractionation, Ceriops decandra, diterpenoids, human pathogens, mangroves

Abstract

The development of resistance towards the antibiotics in use today has been a source of growing concern in the modern healthcare system around the world. To counter this major threat, there is an urgent need for discovery of new antimicrobials. Many plants, like mangroves, possess highly diversified list of natural phytochemicals which are known to have wide range of bioactivities. These phytochemicals can be good sources for the discovery of new drugs. In this study, we report the partial phytochemical characterization and antimicrobial activities of a semi-purified fraction isolated from the wood tissue of Ceriops decandra, a mangrove plant. This fraction named CD-3PM was chromatographically separated from C. decandra wood extract and was subjected to different spectral analyses to determine its partial chemical nature. The structural investigation indicates the presence of two diterpenoids, i) 3β, 13β-Dihydroxy-8-abietaen-7-one and ii) 3β-Hydroxy-8,13-abietadien-7-one in the CD-3PM fraction. The antimicrobial potential of this fraction was evaluated by microdilution-MTT assay against several organisms. Among the nine microorganisms found to be sensitive to the CD-3PM fraction, six organisms are reported to be pathogenic in nature. The CD-3PM fraction with broad spectrum antimicrobial efficacy revealed the presence of two diterpenoids and possesses potential applications in drug discovery process and food processing industries.

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Published

2016-02-09

How to Cite

Simlai, A., Mukherjee, K., Mandal, A., Bhattacharya, K., Samanta, A., & Roy, A. (2016). Partial purification and characterization of an antimicrobial activity from the wood extract of mangrove plant Ceriops decandra. EXCLI Journal, 15, 103–112. https://doi.org/10.17179/excli2015-741

Issue

Vol. 15 (2016)

Section

Original articles

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