Phytochemical evaluation, antioxidant assay, antibacterial activity and determination of cell viability (J774 and THP1 alpha cell lines) of P. sylvestris leaf crude and methanol purified fractions

Authors

  • Dinesh C. Sharma Department of Biosciences, Integral University Lucknow, India-226026
  • Ritu Shukla Department of Biosciences, Integral University Lucknow, India-226026
  • Jasarat Ali Department of Biosciences, Integral University Lucknow, India-226026
  • Swati Sharma Department of Biosciences, Integral University Lucknow, India-226026
  • Priti Bajpai Department of Biosciences, Integral University Lucknow, India-226026
  • Neelam Pathak Department of Biosciences, Integral University Lucknow, India-226026

DOI:

https://doi.org/10.17179/excli2015-689

Keywords:

GC-MS, MPF, MTT assay, ROS

Abstract

Phoenix sylvestris (Arecaceae family) known as Indian Date Palm has been identified as a component of traditional medicine against various ailments. The present study was focused on phytochemical screening of crude hexane, dichloromethane and methanol leaf extracts. The crude extracts showed the presence of alkaloids, flavonoids, and phenols in the plant leaves. In the study methanol extract was found most potent, so this extract was further fractionated by column chromatography and 9 methanol purified fractions (MPFs) were isolated. Most potential MPF8 (20:80 chloroform: methanol ratio fraction) significantly enhanced free radicals and antibacterial activity. The best MIC (Minimum inhibitory concentration) of MPF8 was investigated against M. luteus and E. coli at 1 mg/ml concentration. However, against other bacteria the MIC ranged from 1 mg/ml to 3 mg/ml. The GC-MS analysis showed the presence of many biologically active compounds such as alcohols, flavonoids, aromatic compounds, aldehydes, terpenoids fatty acid methyl esters, and phenolics. Pentadecanoic acid occupied maximum (52 %) area in GC-MS profiling. MPF8 was assayed for in-vitro cytotoxicity by MTT assay which confirms its less cytotoxicity at lower concentration and also significant ROS determination against J774 and THP1 cell lines after 2 and 4 hours.

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Published

2016-02-05

How to Cite

Sharma, D. C., Shukla, R., Ali, J., Sharma, S., Bajpai, P., & Pathak, N. (2016). Phytochemical evaluation, antioxidant assay, antibacterial activity and determination of cell viability (J774 and THP1 alpha cell lines) of P. sylvestris leaf crude and methanol purified fractions . EXCLI Journal, 15, 85–94. https://doi.org/10.17179/excli2015-689

Issue

Vol. 15 (2016)

Section

Original articles

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