Ectopic microRNAs used to preserve human mesenchymal stem cell potency and epigenetics

Authors

  • Navid Ghasemzadeh Department of Biochemistry and Molecular Biology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
  • Fatemeh Pourrajab Department of Biochemistry and Molecular Biology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
  • Ali Dehghani Firoozabadi Yazd Cardiovascular Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
  • Seyedhossein Hekmatimoghaddam Hematology & Oncology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; Department of Laboratory Sciences, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
  • Fatemeh Haghiralsadat Department of Advanced Medical Sciences and Technologies, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

DOI:

https://doi.org/10.17179/excli2018-1274

Keywords:

epigenetic, human mesenchymal stem cells (hMSCs), miRNA, potency, regenerative medicine

Abstract

Human mesenchymal stem cells (hMSCs) have remarkable potential for use in regenerative medicine. However, one of the great challenges is preserving their potency for long time. This study investigated the effect of miRNA ectopic expression on their proliferation and also on the expression level of Parp1 as an epigenetic switch preserving pluripotency in hMSCs. A cationic liposome was prepared as an efficient carrier for miRNA delivery. The miRNA loading efficiency and physical stability of vesicles were measured, and their scanning electron microscopic shapes determined. hMSCs were transfected with miR-302a and miR-34a followed by assessment of their proliferation potency with MTT assay and measurement of the expression of Parp1 by quantitative polymerase chain reaction (QPCR). Cell transfection with miR-302a and miR-34a efficiently and differentially affects the proliferation potency of hMSCs and the expression level of Parp1 as the key epigenetic factor involved in pluripotency. While miR-302a increases Parp1 expression, miR-34a suppresses it significantly, showing differential effects. Our results demonstrated that miRNA-based treatments represent efficient therapeutic systems and hold a great promise for future use in regenerative medicine through modification of hMSC pluripotency and epigenome.

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Published

2018-06-14

How to Cite

Ghasemzadeh, N., Pourrajab, F., Dehghani Firoozabadi, A., Hekmatimoghaddam, S., & Haghiralsadat, F. (2018). Ectopic microRNAs used to preserve human mesenchymal stem cell potency and epigenetics. EXCLI Journal, 17, 576–589. https://doi.org/10.17179/excli2018-1274

Issue

Vol. 17 (2018)

Section

Original articles

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