Bioactive peptide isolated from sesame seeds inhibits cell proliferation and induces apoptosis and autophagy in leukemic cells

Authors

  • Kamolchanok Deesrisak Department of Clinical Microscopy, Faculty of Medical Technology, Mahidol University, Nakhon Pathom 73170, Thailand https://orcid.org/0000-0002-8394-4239
  • Yodying Yingchutrakul Proteomics Research Team, National Omics Center, National Science and Technology Development Agency, Pathum Thani 12120, Thailand https://orcid.org/0000-0003-0701-4205
  • Sucheewin Krobthong Proteomics Research Team, National Omics Center, National Science and Technology Development Agency, Pathum Thani 12120, Thailand
  • Sittiruk Roytrakul Functional Proteomics Technology Laboratory, Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology for Development Agency, Pathum Thani 12120, Thailand https://orcid.org/0000-0003-3696-8390
  • Chawalit Chatupheeraphat Department of Clinical Microscopy, Faculty of Medical Technology, Mahidol University, Nakhon Pathom 73170, Thailand
  • Paweena Subkorn Department of Clinical Microscopy, Faculty of Medical Technology, Mahidol University, Nakhon Pathom 73170, Thailand
  • Usanarat Anurathapan Department of Pediatrics, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand https://orcid.org/0000-0002-2791-7471
  • Dalina Tanyong Department of Clinical Microscopy, Faculty of Medical Technology, Mahidol University, 999 Phuttamonthon sai 4 Road, Salaya, Phuttamonthon, Nakhon Pathom 73170, Thailand; Tel: +662-441-4371-5 Ext. 2836; Fax: +662-441-4380; E-mail: dalina.itc@mahidol.ac.th

DOI:

https://doi.org/10.17179/excli2021-3406

Keywords:

apoptosis, autophagy, bioactive peptide, leukemia, sesame

Abstract

Leukemia is the most common type of hematological malignancies. Several natural products including bioactive peptides have been explored and studied for their anti-leukemic activities. In the present study, anti-leukemic peptide, IGTLILM (IM-7), was isolated and identified from the protein hydrolysate of sesame seeds by reverse phase-solid phase extraction, off-gel fractionation and nano LC-MS/MS. The cytotoxic effects of IM-7 were studied in MOLT-4 and NB4 acute leukemic cell lines using an MTT assay. The induction of apoptosis and autophagy was investigated by flow cytometry using Annexin V-FITC/PI staining and anti-LC3/FITC antibodies, respectively. The mRNA alterations of apoptotic and autophagic-related genes were determined by reverse transcription-quantitative PCR. The present study found that IM-7 inhibited the proliferation of MOLT-4 and NB4 cells in dose-dependent manner, but it showed a minimal effect on healthy mononuclear cells. IM-7 activated apoptosis and autophagy through the upregulation of CASP3, ULK1 and BECN1 and the downregulation of BCL2. In addition, IM-7 enhanced the cytotoxic effect of the anti-leukemic drug, daunorubicin. The findings suggested that IM-7 was potent to suppress the proliferation of MOLT-4 and NB4 leukemic cells and induce apoptosis and autophagy through the regulation of caspase 3-Bcl-2 and ULK1-Beclin1, respectively.

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Published

2021-03-23

How to Cite

Deesrisak, K., Yingchutrakul, Y., Krobthong, S., Roytrakul, S., Chatupheeraphat, C., Subkorn, P., … Tanyong, D. (2021). Bioactive peptide isolated from sesame seeds inhibits cell proliferation and induces apoptosis and autophagy in leukemic cells. EXCLI Journal, 20, 709–721. https://doi.org/10.17179/excli2021-3406

Issue

Vol. 20 (2021)

Section

Original articles

License

Copyright (c) 2021 Kamolchanok Deesrisak, Yodying Yingchutrakul, Sucheewin Krobthong, Sittiruk Roytrakul, Chawalit Chatupheeraphat, Paweena Subkorn, Usanarat Anurathapan, Dalina Tanyong

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