Revealing the astragalin mode of anticandidal action

Authors

  • Marija Ivanov Department of Plant Physiology, Institute for Biological Research “Siniša Stanković” - National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, Serbia; Institute of Microbiology, University Hospital Lausanne and University Hospital Center, Rue du Bugnon 48, Lausanne, Switzerland
  • Abhilash Kannan Institute of Microbiology, University Hospital Lausanne and University Hospital Center, Rue du Bugnon 48, Lausanne, Switzerland
  • Dejan Stojkovic Department of Plant Physiology, Institute for Biological Research “Siniša Stanković” - National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, Serbia
  • Jasmina Glamočlija Department of Plant Physiology, Institute for Biological Research “Siniša Stanković” - National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, Serbia
  • Simona Golič Grdadolnik Laboratory for Molecular Structural Dynamics, National Institute of Chemistry, Hajdrihova ulica 19, 1000 Ljubljana, Slovenia
  • Dominique Sanglard Institute of Microbiology, University Hospital Lausanne and University Hospital Center, Rue du Bugnon 48, Lausanne, Switzerland
  • Marina Soković Department of Plant Physiology, Institute for Biological Research „Siniša Stanković“ - National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, Serbia; Phone: +381 11 207 84 19; Fax: +381 11 2 761 433; E-mail: mris@ibiss.bg.ac.rs

DOI:

https://doi.org/10.17179/excli2020-2987

Keywords:

astragalin, mode of action, antimicrobial, ergosterol biosynthesis, hyphal transition, membrane integrity

Abstract

Due to limited arsenal of systemically available antifungal agents, infections caused by Candida albicans are difficult to treat and the emergence of drug-resistant strains present a major challenge to the clinicians worldwide. Hence further exploration of potential novel and effective antifungal drugs is required. In this study we have explored the potential of a flavonoid, astragalin, in controlling the growth of C. albicans, in both planktonic and biofilm forms by microdilution method; and in regulating the morphological switch between yeast and hyphal growth. Astragalin ability to interfere with membrane integrity, ergosterol synthesis and its role in the regulation of genes encoding for efflux pumps has been addressed. In our study, astragalin treatment produced good antimicrobial and significant antibiofilm activity. Anticandidal activity of astragalin was not related to ERG11 downregulation, neither to direct binding to CYP51 enzyme nor was linked to membrane ergosterol assembly. Instead, astragalin treatment resulted in reduced expression of CDR1 and also affected cell membrane integrity without causing cytotoxic effect on human gingival fibroblast cells. Considering that astragalin-mediated decreased expression of efflux pumps increases the concentration of antifungal drug inside the fungal cells, a combinatorial treatment with this agent could be explored as a novel therapeutic option for candidiasis.

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Published

2020-10-29

How to Cite

Ivanov, M., Kannan, A. ., Stojkovic, D., Glamočlija, J. ., Golič Grdadolnik, S. ., Sanglard, D. ., & Soković, M. . (2020). Revealing the astragalin mode of anticandidal action . EXCLI Journal, 19, 1436–1445. https://doi.org/10.17179/excli2020-2987

Issue

Vol. 19 (2020)

Section

Original articles

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Copyright (c) 2020 Marija Ivanov, Abhilash Kannan, Dejan Stojkovic, Jasmina Glamočlija, Simona Golič Grdadolnik, Dominique Sanglard, Marina Soković

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