Silver nanoparticles restrict microbial growth by promoting oxidative stress and DNA damage

Authors

  • Oluyomi Stephen Adeyemi Laboratory of Theoretical and Computational Biophysics, Ton Duc Thang University, Ho Chi Minh City, Vietnam; Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City, Vietnam; E-mail: oluyomi.stephen.adeyemi@tdtu.edu.vn
  • Emmanuella Oluwatosin Shittu Department of Biochemistry, Medicinal Biochemistry, Nanomedicine & Toxicology Laboratory, Landmark University, PMB 1001, Omu-Aran – 251101, Nigeria
  • Oghenerobor Benjamin Akpor Department of Microbiology, Landmark University, PMB 1001, Omu-Aran – 251101, Nigeria
  • Damilare E. Rotimi Department of Biochemistry, Medicinal Biochemistry, Nanomedicine & Toxicology Laboratory, Landmark University, PMB 1001, Omu-Aran – 251101, Nigeria
  • Gaber El-saber Batiha Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Egypt

DOI:

https://doi.org/10.17179/excli2020-1244

Keywords:

antimicrobial activity, medicinal biochemistry, microbial infection, nanomedicine, nanoparticles

Abstract

Bacterial infections remain a serious health issue; hence there is a need for continuous search for improved antimicrobials. In addition, it is important to understand the antibacterial mechanism of prospective antimicrobials to fully harness their benefits. In this study, the antimicrobial action of silver nanoparticles was investigated. The antimicrobial potential of silver nanoparticles against different strains of bacteria was evaluated after which Escherichia coli and Staphylococcus aureus were selected as model for gram-negative and gram-positive bacteria respectively. Additionally, to determine mechanism of action, some biochemical assays including determination of kynurenine level, DNA fragmentation, lipid peroxidation and antioxidant status were carried out. Results showed that silver nanoparticles caused DNA damage and induced oxidative stress as reflected in elevated nitric oxide production and lipid peroxidation level. In contrast silver nanoparticles increased the antioxidant capacity viz-a-viz, elevated levels of total thiol, superoxide dismutase (SOD), and total antioxidant capacity (TAC) compared to untreated cells. They also initiated inconsistent alteration to the kynurenine pathway. Taken together, the findings indicate that silver nanoparticles exhibited antimicrobial action through the promotion of oxidative stress.

Downloads

Published

2020-04-15

How to Cite

Adeyemi, O. S., Shittu, E. O., Akpor, O. B., Rotimi, D. E., & Batiha, G. E.- saber. (2020). Silver nanoparticles restrict microbial growth by promoting oxidative stress and DNA damage. EXCLI Journal, 19, 492–500. https://doi.org/10.17179/excli2020-1244

Issue

Vol. 19 (2020)

Section

Original articles

License

Authors who publish in this journal agree to the following terms:

  • The authors keep the copyright and grant the journal the right of first publication under the terms of the Creative Commons Attribution license, CC BY 4.0. This licencse permits unrestricted use, distribution and reproduction in any medium, provided that the original work is properly cited.
  • The use of general descriptive names, trade names, trademarks, and so forth in this publication, even if not specifically identified, does not imply that these names are not protected by the relevant laws and regulations.
  • Because the advice and information in this journal are believed to be true and accurate at the time of publication, neither the authors, the editors, nor the publisher accept any legal responsibility for any errors or omissions presented in the publication. The publisher makes no guarantee, express or implied, with respect to the material contained herein.
  • The authors can enter into additional contracts for the non-exclusive distribution of the journal's published version by citing the initial publication in this journal (e.g. publishing in an institutional repository or in a book).

Most read articles by the same author(s)